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mab 2628  (R&D Systems)


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    R&D Systems mab 2628
    Mab 2628, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab+2628/pmc03975727-71-23-25?v=R%26D+Systems
    Average 90 stars, based on 14 article reviews
    mab 2628 - by Bioz Stars, 2026-08
    90/100 stars

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    mab 2628  (R&D Systems)
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    rabbit polyclonal 2628 anti asyn antibodies  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit polyclonal 2628 anti asyn antibodies
    Figure 5. TRAF6 binds <t>aSYN</t> and enhances its ubiquitination with atypical chains. (A) HEK cells were transfected with FLAG–TRAF6 and MYC–aSYN wt or A53T (mut). Lysates were immunoprecipitated with anti-FLAG agarose beads and bound proteins were revealed with anti-MYC and anti-FLAG antibodies. Inputs were tested for the expression of TRAF6 and aSYN proteins. (B) Cells were transfected with HA–ubiquitin, MYC–aSYN (wt or A53T) and FLAG– TRAF6 (wt or DN). For in vivo ubiquitination assay, lysates were immunoprecipitated with anti-MYC antibody. Immunoprecipitates and lysates were analyzed with anti-MYC, anti-FLAG and anti-HA antibodies. (C) HA–ubiquitin mutants in which only the indicated lysine residue is available for chain formation were used. Wt and K0 ubiquitin were included as controls. In vivo ubiquitination of aSYN wt was performed as in (B).
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    Figure 5. TRAF6 binds aSYN and enhances its ubiquitination with atypical chains. (A) HEK cells were transfected with FLAG–TRAF6 and MYC–aSYN wt or A53T (mut). Lysates were immunoprecipitated with anti-FLAG agarose beads and bound proteins were revealed with anti-MYC and anti-FLAG antibodies. Inputs were tested for the expression of TRAF6 and aSYN proteins. (B) Cells were transfected with HA–ubiquitin, MYC–aSYN (wt or A53T) and FLAG– TRAF6 (wt or DN). For in vivo ubiquitination assay, lysates were immunoprecipitated with anti-MYC antibody. Immunoprecipitates and lysates were analyzed with anti-MYC, anti-FLAG and anti-HA antibodies. (C) HA–ubiquitin mutants in which only the indicated lysine residue is available for chain formation were used. Wt and K0 ubiquitin were included as controls. In vivo ubiquitination of aSYN wt was performed as in (B).

    Journal: Human molecular genetics

    Article Title: TRAF6 promotes atypical ubiquitination of mutant DJ-1 and alpha-synuclein and is localized to Lewy bodies in sporadic Parkinson's disease brains.

    doi: 10.1093/hmg/ddq290

    Figure Lengend Snippet: Figure 5. TRAF6 binds aSYN and enhances its ubiquitination with atypical chains. (A) HEK cells were transfected with FLAG–TRAF6 and MYC–aSYN wt or A53T (mut). Lysates were immunoprecipitated with anti-FLAG agarose beads and bound proteins were revealed with anti-MYC and anti-FLAG antibodies. Inputs were tested for the expression of TRAF6 and aSYN proteins. (B) Cells were transfected with HA–ubiquitin, MYC–aSYN (wt or A53T) and FLAG– TRAF6 (wt or DN). For in vivo ubiquitination assay, lysates were immunoprecipitated with anti-MYC antibody. Immunoprecipitates and lysates were analyzed with anti-MYC, anti-FLAG and anti-HA antibodies. (C) HA–ubiquitin mutants in which only the indicated lysine residue is available for chain formation were used. Wt and K0 ubiquitin were included as controls. In vivo ubiquitination of aSYN wt was performed as in (B).

    Article Snippet: Mouse monoclonal (#2647) and rabbit polyclonal (#2628) anti-aSYN antibodies were from Cell Signaling.

    Techniques: Ubiquitin Proteomics, Transfection, Immunoprecipitation, Expressing, In Vivo, Residue

    Figure 6. TRAF6 colocalizes with aSYN in LBs and accumulates in PD post-mortem brains. (A) Cryo-sections of post-mortem brain tissues were taken from healthy individuals and endogenous TRAF6 was visualized by immunohistochemistry. DA neurons were identified with anti-TH antibody. (B) TRAF6 was ana- lyzed by immunohistochemistry in SN of PD patients. LBs were visualized with anti-aSYN antibody. (C) Total RNA was extracted from SN of six PD and three control brains. TRAF6 mRNA was measured by qPCR relative to b-actin. Significance between PD and control group was calculated with t-test.

    Journal: Human molecular genetics

    Article Title: TRAF6 promotes atypical ubiquitination of mutant DJ-1 and alpha-synuclein and is localized to Lewy bodies in sporadic Parkinson's disease brains.

    doi: 10.1093/hmg/ddq290

    Figure Lengend Snippet: Figure 6. TRAF6 colocalizes with aSYN in LBs and accumulates in PD post-mortem brains. (A) Cryo-sections of post-mortem brain tissues were taken from healthy individuals and endogenous TRAF6 was visualized by immunohistochemistry. DA neurons were identified with anti-TH antibody. (B) TRAF6 was ana- lyzed by immunohistochemistry in SN of PD patients. LBs were visualized with anti-aSYN antibody. (C) Total RNA was extracted from SN of six PD and three control brains. TRAF6 mRNA was measured by qPCR relative to b-actin. Significance between PD and control group was calculated with t-test.

    Article Snippet: Mouse monoclonal (#2647) and rabbit polyclonal (#2628) anti-aSYN antibodies were from Cell Signaling.

    Techniques: Immunohistochemistry, Control